Cryptochrome-2 (CRY2), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Cryptochrome-2 (CRY2) , partial

Product Gene Name

CRY2 recombinant protein

Product Synonym Gene Name

CRY2

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

NTIKEPGLCP SVSSNDQQVP SAVRYNGSKR VKPEEEEERD MKKSRGFDER ELFSTAESSS SSSVFFVSQS CSLASEGKNL EGIQDSSDQI TTSLGKNGCK

Sequence Positions

513-612; Fragment at the C-terminal.

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

69,457 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Cryptochrome

NCBI Accession #

NP_171935.1

NCBI GI #

15219720

NCBI GenBank Nucleotide #

NM_100320.4

NCBI GeneID

839529

NCBI Official Full Name

cryptochrome 2

NCBI Official Symbol

CRY2

NCBI Official Synonym Symbols

AT-PHH1; ATCRY2; cryptochrome 2; CRYPTOCHROME 2 APOPROTEIN; F19P19.14; F19P19_14; FHA; PHH1

NCBI Protein Information

cryptochrome 2

NCBI Summary

Blue light receptor mediating blue-light regulated cotyledon expansion and flowering time. Positive regulator of the flowering-time gene CONSTANS. This gene possesses a light-induced CNT2 N-terminal homodimerisation domain.Involved in blue-light induced stomatal opening. Involved in triggering chromatin decondensation. An 80-residue motif (NC80) is sufficient to confer CRY2's physiological function. It is proposed that the PHR domain and the C-terminal tail of the unphosphorylated CRY2 form a ''closed'' conformation to suppress the NC80 motif in the absence of light. In response to blue light, the C-terminal tail of CRY2 is phosphorylated and electrostatically repelled from the surface of the PHR domain to form an ''open'' conformation, resulting in derepression of the NC80 motif and signal transduction to trigger photomorphogenic responses. Cry2 phosphorylation and degradation both occur in the nucleus.

UniProt Gene Name

CRY2

UniProt Synonym Gene Names

AtPHH1

UniProt Protein Name

Cryptochrome-2

UniProt Synonym Protein Names

Blue light photoreceptor

UniProt Primary Accession #

Q96524

UniProt Secondary Accession #

Q42549; Q42603; Q42604; Q56ZL8; Q696X6; Q696X8; Q696Z7; B0LQ23; B0LQ24; B0LQ25; B0LQ29

UniProt Related Accession #

Q96524

UniProt Comments

Photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Photoexcited cryptochromes interact with signaling partner proteins to alter gene expression at both transcriptional and post-translational levels and, consequently, regulate the corresponding metabolic and developmental programs (PubMed:21841916). Blue-light absorbing flavoprotein that activates reversible flavin photoreduction via an electron transport chain comprising a tryptophan triad (W-321, W-374 and W-397), or via an alternative electron transport that involves small metabolites, including NADPH, NADH, and ATP. The half-life of the activated signaling state is about 16 minutes (PubMed:25428980, PubMed:23398192). Perceives low blue light (LBL) and responds by directly contacting two bHLH transcription factors, PIF4 and PIF5, at chromatin on E-box variant 5'-CA[CT]GTG-3' to promote their activity and stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation and hyponastic growth in low blue light) (PubMed:26724867, PubMed:19558423). In response to blue light, binds to CIB proteins (e.g. BHLH63/CIB1 and BHLH76/CIB5) to activates transcription and floral initiation (PubMed:24130508). Mediates blue light-induced gene expression, floral initiation and hypocotyl elongation through the interaction with SPA1 that prevents formation of SPA1/COP1 complex but stimulates COP1 binding, and thus inhibits COP1-mediated degradation of transcription factors (e.g. CO and HY5) (PubMed:21514160, PubMed:21511872, PubMed:16093319). Promotes flowering time in continuous light (LL) (PubMed:21296763). Involved in shortening the circadian clock period, especially at 27 degrees Celsius, in blue light (BL). Required to maintain clock genes expression rhythm (PubMed:23511208). Triggers nuclear accumulation of ROS in response to blue light illumination (PubMed:26179959). Involved in blue light-dependent stomatal opening, transpiration and inhibition of stem and root growth, probably by regulating abscisic acid (ABA) (PubMed:22147516, PubMed:16093319, PubMed:16703358, PubMed:9482948, PubMed:9565033). Regulates the timing of flowering by promoting the expression of 'FLOWERING LOCUS T' (FT) in vascular bundles. Negatively regulated by 'FLOWERING LOCUS C' (FLC) (PubMed:14605222, PubMed:17259260). General positive regulator of reversible low light-induced chromatin decompaction (PubMed:20935177). Involved in triggering chromatin decondensation during floral transition (PubMed:17470059). Together with phototropins, involved in phototropism regulation by various blue light fluence; blue light attenuates phototropism in high fluence rates (100 µmol.m-2.s-1) but enhances phototropism in low fluence rates (<1.0 µmol.m-2.s-1) (PubMed:12857830). The effect of near-null magnetic field on flowering is altered by changes of blue light cycle and intensity in a CRY1/CRY2-dependent manner (PubMed:26095447). Involved in the strigolactone signaling that regulates hypocotyl growth in response to blue light (PubMed:24126495).