Immunogen
                                                
                                                
                                                    KLH-conjugated synthetic peptide conserved in isoform 1,2,3,4,7,8,10,11,14,20,25,30,32 of Arabidopsis thaliana CDPK kinases                                                
                                             
                                                                                    
                                                                                    
                                                                                    
                                                
                                                    Purity
                                                
                                                
                                                    Immunogen affinity purified serum in PBS pH 7.4.                                                
                                             
                                                                                    
                                                                                    
                                                
                                                    Reconstitution
                                                
                                                
                                                    For reconstitution add 50 µl of sterile water                                                
                                             
                                                                                    
                                                
                                                    Storage
                                                
                                                
                                                    Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.                                                
                                             
                                                                                    
                                                
                                                    Application
                                                
                                                
                                                    Western blot (WB), Immunoprecipitation (IP)                                                
                                             
                                                                                    
                                                
                                                    Recommended dilution
                                                
                                                
                                                    1 : 1000 (WB)                                                
                                             
                                                                                    
                                                
                                                    Expected | apparent MW
                                                
                                                
                                                    52-55 kDa