Cas9 | CRISPR-associated endonuclease 9 (monoclonal)

Inquiry

Cat: PA00017

Size: 50 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Cas9 from Streptococcus pyogenes

Datasheet:

Product Info

Immunogen

Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.

Host

Mouse

Clonality

Monoclonal

Purity

Total IgG. Protein A purified in PBS. Contains 0.02 % sodium azide.

Format

Liquid

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For long term storage -80°C is recommended. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 200 (IF), (IP), 1 : 1000-1 : 5000 (WB)

Expected | apparent MW

Depends upon a MW of a protein which is a fusion partner

Reactivity

Confirmed reactivity

Cas9 from Streptococcus pyogenes

Description

Cas9 (CRISPR-Associated endonucase 9) is an RNA-directed DNA endonucase associated with the clustered regularly interspersed short palindromic repeat (CRISPR) type II adaptive immune system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein is used as a genomic engineering tool to induce site-directed double-strand breaks in DNA. Alternative name :Csn1.

For research use only, not for clinical use.