Bifunctional dihydrocamalexate synthase/camalexin synthase (CYP71B15), Recombinant Protein
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Bifunctional dihydrocamalexate synthase/camalexin synthase (CYP71B15), Recombinant Protein

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Cat: RP08869
Size: 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Bifunctional dihydrocamalexate synthase/camalexin synthase (CYP71B15), partial
Product Gene Name
CYP71B15 recombinant protein
Product Synonym Gene Name
CYP71B15
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MSVFLCFLVL LPLILIFLNV LKPSKYKLPP GPKKLPIIGN LHQRRTLHPR NRRNLAEMYG PVALLQYGFV PVVAISSKEA AEEVLKINDL ECCSRPEAAG MRATFYNFKD IGMAPFGDEW SLMRKLSVVE LFSVKKLQSF KYIIEEENNL CVKKLSEFAT RQSPVNLERA IFTLVGNIVC RIGYGINLYE CDFFEADRVV DLVLKAEAVI RETVFSDFFP GRIGRFIDCI SGQNRRLKNN FSVVDTFFQN VLNEHLKPGR ESSTIVDLMI DMKKKQENDG DALKFTTDHL KGMISDIFVA GIGGVAGITL WGMTELIRNP RVMKKVQDEI RTTLGDKKER IKEEDLNQLH YFKLVVKETL RLHPTTPLLL PRQTMSHIKI QGYDVPAKTQ ILVNVYAMGR DPKLWENADE FNPDRFLDSS VDFKGKNYEF IPFGSGRRIC PGMTMGTILV EMALLNLLYF FDWGLAKQEE AKEIINGEEN FLAFFQVLHH
Sequence Positions
1-490
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
56,061 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Bifunctional dihydrocamalexate synthase/camalexin synthase

NCBI/Uniprot Data

NCBI Accession #
NP_189318.1
NCBI GI #
15231632
NCBI GenBank Nucleotide #
NM_113595.4
NCBI GeneID
822298
NCBI Official Full Name
Cytochrome P450 superfamily protein
NCBI Official Symbol
PAD3
NCBI Official Synonym Symbols
CYP71B15; PHYTOALEXIN DEFICIENT 3
NCBI Protein Information
Cytochrome P450 superfamily protein
NCBI Summary
Mutations in pad3 are defective in biosynthesis of the indole derived phytoalexin camalexin. Encodes a cytochrome P450 enzyme that catalyzes the conversion of dihydrocamalexic acid to camalexin.
UniProt Gene Name
CYP71B15
UniProt Synonym Gene Names
PAD3
UniProt Protein Name
Bifunctional dihydrocamalexate synthase/camalexin synthase
UniProt Synonym Protein Names
Cytochrome P450 71B15; Dihydrocamalexate:NADP(+) oxidoreductase (decarboxylating); Protein PHYTOALEXIN DEFICIENT 3
UniProt Primary Accession #
Q9LW27
UniProt Secondary Accession #
Q541X4
UniProt Related Accession #
Q9LW27
UniProt Comments
Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.

For research use only, not for clinical use.