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Beta-glucosidase 21 (BGLU21), Recombinant Protein
Cat: RP06506
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Beta-glucosidase 21 (BGLU21)
Product Gene Name
BGLU21 recombinant protein
Product Synonym Gene Name
BGLU21
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
VDDPVCPATS KLSRASFPNG FLFGTATAAF QVEGAINETC RGPALWDIYC RRNPERCSGD HADVAVDFFH RYKEDIQLMK NLNTDAFRLS IAWSRIFPHG RKEKGVSQAG VQFYHELIDE LLKNGIVPFV TVFHWDTPQD LEDEYGGFLS QNIVKDFREY ADYVFTEYGG KVKNWITFNE PWVFAHAGYD LGKKAPGRCS RYVPGCEDRE GQSGKEAYLV SHNLLNAHAE AVEVFRQKVK GGKIGIAHSP AWFEPHDLKD SNDAPTVSRV LDFMLGWHLE PTTSGDYPQI MKDLLGYRLP QFTAAQKAKL KDSTDFVGLN YYTSTFSNYN EKPDPSKPSW KQDSLVSWEP KNVDHSAIGS MPLTAALPVY AKGFRKLLKY IKDKYANPEI MIMENGYGDK LGTTDSVDVG TADHNRKYYL QRHLLAMNEA ICIDKVRVTG YFVWSLLDNF EWQDGYKNRF GLYYVDFKNN LTRYEKESAK YYKDFLAQGV RPSALKRDEL
Sequence Positions
25-524, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
59,493 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Beta-glucosidase
NCBI/Uniprot Data
NCBI Accession #
NP_176801.1
NCBI GI #
15219605
NCBI GenBank Nucleotide #
NM_105298.4
NCBI GeneID
842944
NCBI Official Full Name
Glycosyl hydrolase superfamily protein
NCBI Official Symbol
BGLU21
NCBI Official Synonym Symbols
T6J19.2
NCBI Protein Information
Glycosyl hydrolase superfamily protein
NCBI Summary
BGLU21 encodes a beta-glucosidase that has a high level of activity against the naturally occuring secondary metabolite scopolin. Recombinant BGLU21 produced in insect cells shows lower levels of activity using the structurally similar substrates esculin (33%) and 4-MU-glucoside (9%). BGLU21 is glycosylated in planta and has a C-terminal ER retention signal. Transcript analysis as well as protein extraction and western blotting from various plant organs suggest that BGLU21 is only present in roots and seedlings. Transcript levels increase in response to cold, auxin (2,4-D), and methyl jasmonate treatments.
UniProt Gene Name
BGLU21
UniProt Synonym Gene Names
PSR3.2; AtBGLU21
UniProt Protein Name
Beta-glucosidase 21
UniProt Synonym Protein Names
Protein PHOSPHATE STARVATION-RESPONSE 3.2
UniProt Primary Accession #
Q9C525
UniProt Secondary Accession #
O23656; Q3ECH6
UniProt Related Accession #
Q9C525
UniProt Comments
Beta-D-glucosidase active on scopolin >> esculin >> 4-MU-glucoside > DIMBOA-glucoside. No activity with pNP-glucoside, oNP-glucoside and sinigrin as substrates.
For research use only, not for clinical use.