Auxin-binding protein 1 (ERABP1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Auxin-binding protein 1 (ERABP1)

Product Gene Name

ERABP1 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

APCPINGLPI VRNISDLPQD NYGRPGLSHM TVAGSVLHGM KEVEIWLQTF APGSETPIHR HSCEEVFVVL KGSGTLYLAE THGNFPGKPI EFPIFANSTI HIPINDAHQV KNTGHEDLQV LVIISRPPIK IFIYEDWFMP HTAARLKFPY YWDEQCIQES QKDEL

Sequence Positions

34-198, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

22,044 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

NP_192207.1

NCBI GI #

15236229

NCBI GenBank Nucleotide #

NM_116532.3

NCBI GeneID

828120

NCBI Official Full Name

endoplasmic reticulum auxin binding protein 1

NCBI Official Symbol

ABP1

NCBI Official Synonym Symbols

ABP; AT-ERABP1; endoplasmic reticulum auxin binding protein 1; T4I9.14; T4I9_14

NCBI Protein Information

endoplasmic reticulum auxin binding protein 1

NCBI Summary

Auxin binding protein involved in cell elongation and cell division. ABP1 is ubiquitinated in vitro and in planta by AtRma2.

UniProt Gene Name

ERABP1

UniProt Synonym Gene Names

ABP1

UniProt Protein Name

Auxin-binding protein 1

UniProt Primary Accession #

P33487

UniProt Secondary Accession #

Q8LBB3

UniProt Related Accession #

P33487

UniProt Comments

Auxin receptor that controls cell elongation and cell division (PubMed:9804548, PubMed:11297513). Involved in embryonic morphogenesis (PubMed:11297513). Acts on the cell cycle, endocycle, cell plate formation, and cell expansion and contributes to the control of auxin-related gene expression (PubMed:18952781). Controls root meristem size and mediates auxin responsiveness (PubMed:19777056). Involved in activation of ROP GTPases in response to auxin and regulation of clathrin-mediated endocytosis in roots (PubMed:20887895, PubMed:22683261). Acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis (PubMed:20887896, PubMed:25922490). Upon auxin binding, restricts the internalization of PIN proteins by inhibiting clathrin-mediated endocytosis (PubMed:20887896, PubMed:25922490). Involved in the regulation of polar auxin transport (PubMed:21223392). Behaves as a negative regulator of the SCF(TIR1/AFB) signaling pathway, protecting AUX/IAA repressors from degradation (PubMed:24051655). Regulates the expression of cell wall remodeling genes via an SCF(TIR1/AFB)-dependent pathway (PubMed:24424095). Involved in the modulation of hemicellulose xyloglucan structure (PubMed:24424095). Required for rapid auxin-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses (PubMed:25409144). Involved in the shade avoidance response (PubMed:24052532). Forms with TMK1 a cell surface auxin perception complex that activates ROP signaling pathways (PubMed:24578577). ABP1 sensing of auxin is important for the ABP1-TMK1 complex formation (PubMed:24578577). Interacts functionally with phytochrome to regulate growth (PubMed:25392478).