AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

Cat: PA00519

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Dionaea muscipula, Echinochloa crus/galli, Escherichia coli, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea mays Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Ultrastructure Expansion Microscopy (U-ExM), Western blot (WB)

Recommended dilution

1 : 100 (IF), 1 : 5000 (BN-PAGE), 1: 2500 (U-ExM), 1 : 2000-1 : 5 000 (WB)

Expected | apparent MW

53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Dionaea muscipula, Echinochloa crus/galli, Escherichia coli, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea mays Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria

Predicted reactivity

Acinetobacter baumannii, Algae, Bacillus subtilis, Brassica napus, Cannabis sativa, Clostridioides difficile, Cyanobacteria, E.coli K-12, Galdieria sulphuraria, Heliomicrobium modesticaldum Ice1, Manihot esculenta, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Zosteria marina, Yrsinia sp.

Not reactive in

Archeal V-type ATP synthase

Additional information

The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody is used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested,Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness is 2 mm with 4.5-16 % gradient. Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Description

ATP synthase is a general-purpose enzyme that synthesizes ATP from ADP and phosphate using energy stored in a transmembrane ion gradient.

For research use only, not for clinical use.