ATG9 | Autophagy-related protein 9 (C-terminal)

Inquiry

Cat: PA00409

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana ATG9 protein sequence, UniProt: Q8RUS5, TAIR: AT2G31260

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 10 000 (WB)

Expected | apparent MW

99,5 | 100 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Aquilegia coerulea, Brassica napus, Capsella rubella, Daucus carota, Eutrema salsugineum, Gossypium hirsutum, Juglans regia, Lactuca sativa, Noccaea caerulescens, Theobroma cacao, Trema orientale

Not reactive in

Nicotiana tabacum

Additional information

Plant ATG9 is most likely N-glycosylated, and glycosylated membrane proteins can migrate as more than one form in SDS-PAGE (smear); the protein most likely forms functional dimers which could be detected also after western blotting. Usage of microsomal fraction is highly recommended.

Description

Autophagy-related protein 9 (ATG9) is an essential protein in plant autophagy and plays a crucial role in plant nutrient cycling. Alternative name :AtAPG9.

For research use only, not for clinical use.