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ATG5 | Autophagy protein 5
Cat: PA01218
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:
Product Info
Immunogen
Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Solanum lycopersicum, Solanum tuberosum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Additional information
In wild-type plants almost all ATG5 is conjugated to ATG12.This item can be sold containing ProClin
Description
ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in regulating the negative feedback loop of the NPR1-dependent salicylic acid (SA) signaling pathway, limiting aging and immune-related programmed cell death (PCD) in plants, and complete proteolysis of chloroplast matrix proteins during damaged peroxisome degradation in aging leaves. Alias: Protein autophagy 5,AtAPG5.
For research use only, not for clinical use.