Peanut lectin (PNA) is purified by affinity chromatography and consists of four subunits, each of about 27,000 subunits. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It is carbohydrate-specific for Galβ3GalNAc with galactose-eluting sugar. Galactose and lactose are poor inhibitors, while disaccharide galactose beta (1->3)GalNAc and defatalized human red blood cell substance N (t antigen, present in many M and N blood groups) are potent inhibitors of PNA. Although peanut extract does not agglutinate untreated or trypsin-treated human red blood cells, PNA is known to agglutinate neuraminidase-treated human red blood cells. Peanut lectin (PNA) is labeled with fluorescein isothiocyanate (FITC) and combined with appropriate amounts of fluorescent dyes to provide the best staining properties for this lectin.
For research use only, not for clinical use.