Adenylyltransferase and sulfurtransferase MOCS3 2 (MOCS3-2), Recombinant Protein
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Adenylyltransferase and sulfurtransferase MOCS3 2 (MOCS3-2), Recombinant Protein

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Cat: RP10693
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Zea mays (Maize)
Datasheet:

Product Info

Full Product Name
Recombinant Zea mays Adenylyltransferase and sulfurtransferase MOCS3 2 (MOCS3-2)
Product Gene Name
MOCS3-2 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MLRTRGPGSI LDPPLGGRRE AIERELKKLR AEREELDGRI RLLESQLEAG PAGFNGAAAG KGVGDDACGG SGACQSRVGN GFAPDGSLPA DMIYRYSRHL LLPDFGVEGQ RKLSQSSILV VGAGGLGSPL ALYLAACGVG CLGIVDGDDV ELNNLHRQII HKEAYVGQSK VKSAADACRE INSAIKVVEH HHTLKPCNAL EIARKYDIVV DATDNLPTRY MISDCCVLLN KPLVSGAALG LEGQLTVYHH NGSPCYRCLF PTPPPVAACQ RCSDSGVLGV VPGVIGCLQA LEAIKVATGV GEPLCGRMLL FDALSARIRV VKLRGSSPDC THCGENSVFT EEDFQKFDYE SFTQSPMSDK AAASVNVLPE SARITCREYK KLADDGEPHL LLDVRPAHHF QIASISPSHN IPLSMLEEKL PALEASLKEA GEGSALVVLC RRGNDSQRAV QLLREKGFAN AKDIIGGLQA WGQDVDPDFP VY
Sequence Positions
1-482, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
51,539 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_001130855.1
NCBI GI #
212274603
NCBI GenBank Nucleotide #
NM_001137383.1
NCBI GeneID
100191959
NCBI Official Full Name
adenylyltransferase and sulfurtransferase MOCS3-2
NCBI Official Symbol
MOCS3-2
NCBI Official Synonym Symbols
UBA4-2; GRMZM2G157836; GRMZM5G881641
NCBI Protein Information
adenylyltransferase and sulfurtransferase MOCS3-2
UniProt Gene Name
MOCS3-2
UniProt Protein Name
Adenylyltransferase and sulfurtransferase MOCS3-2
UniProt Synonym Protein Names
Sulfur carrier protein MOCS2A sulfurtransferase 2
UniProt Primary Accession #
B4FAT0
UniProt Related Accession #
B4FAT0
UniProt Comments
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

For research use only, not for clinical use.