ABI1 | Abscisic acid insensitive 1
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ABI1 | Abscisic acid insensitive 1

Cat: PA01601
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
5 µg (IP for a 200 ul of a cell extract), 3 µg (WB)
Expected | apparent MW
47,5 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal (non stressed) conditions, The antibody detects both, recombinant and endogenous ABI1 proteins.,Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody

Description

ABI1 is a key component and inhibitor of abscisic acid (ABA) signaling pathway. It regulates many ABA responses, such as stomatal closure, plasma membrane permeation water, drought-induced resistance and root formation, response to glucose, strong light stress, and inhibition of seed germination and vegetative growth. It is expressed in seeds and seedlings. Confined to lateral root cap and columnar cells of the root. Low temperature, drought, high salt, ABA and ethylene induction. Aba-dependent stimuli activate/inhibit SnRK2.6/SRK2E/OST1. Alias :ABI1, ABA-Insensitive 1,AtABI1, protein phosphatase 2C56, AtPP2C56, PP2C56.

For research use only, not for clinical use.