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4-substituted benzoates-glutamate ligase GH3.12 (GH3.12), Recombinant Protein
Cat: RP06689
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana 4-substituted benzoates-glutamate ligase GH3.12 (GH3.12) , partial
Product Gene Name
GH3.12 recombinant protein
Product Synonym Gene Name
GH3.12
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
65,128 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
4-substituted benzoates-glutamate ligase
NCBI/Uniprot Data
NCBI Accession #
NP_001330076.1
NCBI GI #
1063731602
NCBI GenBank Nucleotide #
NM_001343269.1
NCBI GeneID
831173
NCBI Official Full Name
Auxin-responsive GH3 family protein
NCBI Official Symbol
PBS3
NCBI Official Synonym Symbols
AtGH3.12; AVRPPHB SUSCEPTIBLE 3; GDG1; GH3-LIKE DEFENSE GENE 1; GH3.12; GRETCHEN HAGEN 3.12; HOPW1-1-INTERACTING 3; T22N19.5; WIN3
NCBI Protein Information
Auxin-responsive GH3 family protein
NCBI Summary
Encodes an enzyme capable of conjugating amino acids to 4-substituted benzoates. 4-HBA (4-hydroxybenzoic acid) and pABA (4-aminobenzoate) may be targets of the enzyme in Arabidopsis, leading to the production of pABA-Glu, 4HBA-Glu, or other related compounds. This enzyme is involved in disease-resistance signaling. It is required for the accumulation of salicylic acid, activation of defense responses, and resistance to Pseudomonas syringae. Salicylic acid can decrease this enzyme's activity in vitro and may act as a competitive inhibitor. Expression of PBS3/GH3.12 can be detected in cotyledons, true leaves, hypocotyls, and occasionally in some parts of roots from 10-day-old seedlings. No expression has been detected in root, stem, rosette or cauline leaves of mature 4- to 5-week-old plants.
UniProt Gene Name
GH3.12
UniProt Synonym Gene Names
GDG1; PBS3; WIN3; AtGH3-12
UniProt Protein Name
4-substituted benzoates-glutamate ligase GH3.12
UniProt Synonym Protein Names
Auxin-responsive GH3-like protein 12; AtGH3-12; Protein GH3-LIKE DEFENSE GENE 1; Protein GRETCHEN HAGEN 3.12; Protein HOPW1-1-INTERACTING 3; Protein avrPPHB SUSCEPTIBLE 3
UniProt Primary Accession #
Q9LYU4
UniProt Related Accession #
Q9LYU4
UniProt Comments
Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).
For research use only, not for clinical use.