3-phosphoinositide-dependent protein kinase 1 (PDPK1), Recombinant Protein
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3-phosphoinositide-dependent protein kinase 1 (PDPK1), Recombinant Protein

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Cat: RP03950
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 3-phosphoinositide-dependent protein kinase 1 (PDPK1)
Product Gene Name
PDPK1 recombinant protein
Product Synonym Gene Name
PDPK1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MLAMEKEFDS KLVLQGNSSN GANVSRSKSF SFKAPQENFT SHDFEFGKIY GVGSYSKVVR AKKKETGTVY ALKIMDKKFI TKENKTAYVK LERIVLDQLE HPGIIKLYFT FQDTSSLYMA LESCEGGELF DQITRKGRLS EDEARFYTAE VVDALEYIHS MGLIHRDIKP ENLLLTSDGH IKIADFGSVK PMQDSQITVL PNAASDDKAC TFVGTAAYVP PEVLNSSPAT FGNDLWALGC TLYQMLSGTS PFKDASEWLI FQRIIARDIK FPNHFSEAAR DLIDRLLDTE PSRRPGAGSE GYVALKRHPF FNGVDWKNLR SQTPPKLAPD PASQTASPER DDTHGSPWNL THIGDSLATQ NEGHSAPPTS SESSGSITRL ASIDSFDSRW QQFLEPGESV LMISAVKKLQ KITSKKVQLI LTNKPKLIYV DPSKLVVKGN IIWSDNSNDL NVVVTSPSHF KICTPKKVLS FEDAKQRASV WKKAIETLQN R
Sequence Positions
1-491, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
54,711 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
3-phosphoinositide-dependent protein kinase

NCBI/Uniprot Data

NCBI Accession #
NP_568138.1
NCBI GI #
18414583
NCBI GenBank Nucleotide #
NM_120533.5
NCBI GeneID
830330
NCBI Official Full Name
3'-phosphoinositide-dependent protein kinase 1
NCBI Official Symbol
PDK1
NCBI Official Synonym Symbols
3'-phosphoinositide-dependent protein kinase 1; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1; ATPDK1; T32M21.110; T32M21_110
NCBI Protein Information
3'-phosphoinositide-dependent protein kinase 1
NCBI Summary
Encodes 3-phosphoinositide-dependent protein kinase that contains pleckstrin homology domain and binds 3-phosphoinositides. It activates the protein kinase AGC2-1 in a phosphatidic acid dependent manner. Phosphorylates S6K1. Interacts with PID, and transphosphorylation by PDK1 increases PID autophosphorylation.
UniProt Gene Name
PDPK1
UniProt Synonym Gene Names
PDK1; AtPDK1
UniProt Protein Name
3-phosphoinositide-dependent protein kinase 1
UniProt Primary Accession #
Q9XF67
UniProt Secondary Accession #
Q9LZ74
UniProt Related Accession #
Q9XF67
UniProt Comments
May couple lipid signals to the activation-loop phosphorylation of several protein kinases of the so-called AGC kinase family. Interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. May play a general role in signaling processes controlling the pathogen/stress response, polar auxin transport and development. Transphosphorylates the AGC protein kinases OXI1/AGC2-1, PK1/S6K1, PK19/S6K2 and PID resulting in their activation.

For research use only, not for clinical use.