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3-epi-6-deoxocathasterone 23-monooxygenase (ROT3), Recombinant Protein
Cat: RP03443
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana 3-epi-6-deoxocathasterone 23-monooxygenase (ROT3) , partial
Product Gene Name
ROT3 recombinant protein
Product Synonym Gene Name
ROT3
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
59,356 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
3-epi-6-deoxocathasterone 23-monooxygenase
NCBI/Uniprot Data
NCBI Accession #
NP_568002.1
NCBI GI #
18419825
NCBI GenBank Nucleotide #
NM_119801.4
NCBI GeneID
829790
NCBI Official Full Name
Cytochrome P450 superfamily protein
NCBI Official Symbol
ROT3
NCBI Official Synonym Symbols
AP22.10; AP22_10; CYP90C1; ROTUNDIFOLIA 3
NCBI Protein Information
Cytochrome P450 superfamily protein
NCBI Summary
Encodes a cytochrome P-450 gene that is involved in leaf blade expansion by controlling polar cell expansion in the leaf length direction. Member of the CYP90C CYP450 family. ROT3 was shown to be involved in brassinosteroid biosynthesis, most likely in the conversion step of typhasterol (TY) to castasterone (CS). As 6-deoxo-CS was unable to restore the phenotype of rot3-1, it has been postulated that ROT3 might be specifically involved in the conversion of TY to CS in the C6-oxidation pathway of brassinolide. Recently, CYP90C1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates).
UniProt Gene Name
ROT3
UniProt Synonym Gene Names
CYP90C1
UniProt Protein Name
3-epi-6-deoxocathasterone 23-monooxygenase
UniProt Synonym Protein Names
Cytochrome P450 90C1; Protein ROTUNDIFOLIA 3
UniProt Primary Accession #
Q9M066
UniProt Secondary Accession #
O23242; Q058J8; Q0WS16
UniProt Related Accession #
Q9M066
UniProt Comments
C-23 hydroxylase involved in brassinosteroids biosynthesis. Converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol. These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone. Functionally redundant with CYP90D1.
For research use only, not for clinical use.