3-epi-6-deoxocathasterone 23-monooxygenase (CYP90D1), Recombinant Protein
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3-epi-6-deoxocathasterone 23-monooxygenase (CYP90D1), Recombinant Protein

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Cat: RP08030
Size: 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 3-epi-6-deoxocathasterone 23-monooxygenase (CYP90D1), partial
Product Gene Name
CYP90D1 recombinant protein
Product Synonym Gene Name
CYP90D1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MDTSSSLLFF SFFFFIIIVI FNKINGLRSS PASKKKLNDH HVTSQSHGPK FPHGSLGWPV IGETIEFVSS AYSDRPESFM DKRRLMYGRV FKSHIFGTAT IVSTDAEVNR AVLQSDSTAF VPFYPKTVRE LMGKSSILLI NGSLHRRFHG LVGSFLKSPL LKAQIVRDMH KFLSESMDLW SEDQPVLLQD VSKTVAFKVL AKALISVEKG EDLEELKREF ENFISGLMSL PINFPGTQLH RSLQAKKNMV KQVERIIEGK IRKTKNKEED DVIAKDVVDV LLKDSSEHLT HNLIANNMID MMIPGHDSVP VLITLAVKFL SDSPAALNLL TEENMKLKSL KELTGEPLYW NDYLSLPFTQ KVITETLRMG NVIIGVMRKA MKDVEIKGYV IPKGWCFLAY LRSVHLDKLY YESPYKFNPW RWQERDMNTS SFSPFGGGQR LCPGLDLARL ETSVFLHHLV TRFRWIAEED TIINFPTVHM KNKLPIWIKR I
Sequence Positions
1-491
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
56,153 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_001327080.1
NCBI GI #
1063711321
NCBI GenBank Nucleotide #
NM_001338068.1
NCBI GeneID
820582
NCBI Official Full Name
cytochrome P450, family 90, subfamily D, polypeptide 1
NCBI Official Symbol
CYP90D1
NCBI Official Synonym Symbols
''cytochrome P450; cytochrome P450; family 90; polypeptide 1; polypeptide 1''; subfamily D
NCBI Protein Information
cytochrome P450, family 90, subfamily D, polypeptide 1
NCBI Summary
Encodes a cytochrome P-450 gene that is involved in brassinosteroid biosynthesis, most likely in the conversion step of teasterone (TE) to 3-dehydroteasterone (3DT), and/or 6-deoxoteasterone (6-deoxoTE) to 6-deoxo-3-dehydroteasterone (6-deoxo3DT); or the conversion of cathasterone (CT) to TE, and/or 6-deoxocathasterone (6-deoxoCT) to 6-deoxoTE. Recently, CYP90D1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates). Member of the CYP90C CYP450 family. Similar to Cytochrome P450 90C1 (ROT3).
UniProt Gene Name
CYP90D1
UniProt Protein Name
3-epi-6-deoxocathasterone 23-monooxygenase
UniProt Synonym Protein Names
Cytochrome P450 90D1
UniProt Primary Accession #
Q94IA6
UniProt Secondary Accession #
Q9LIC5
UniProt Related Accession #
Q94IA6
UniProt Comments
C-23 hydroxylase involved in brassinosteroids biosynthesis. Converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol. These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone. Functionally redundant with CYP90C1.

For research use only, not for clinical use.