2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD), Recombinant Protein
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2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD), Recombinant Protein

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Cat: RP01028
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD)
Product Gene Name
ISPD recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
SSSTGFDNSN VVVKEKSVSV ILLAGGQGKR MKMSMPKQYI PLLGQPIALY SFFTFSRMPE VKEIVVVCDP FFRDIFEEYE ESIDVDLRFA IPGKERQDSV YSGLQEIDVN SELVCIHDSA RPLVNTEDVE KVLKDGSAVG AAVLGVPAKA TIKEVNSDSL VVKTLDRKTL WEMQTPQVIK PELLKKGFEL VKSEGLEVTD DVSIVEYLKH PVYVSQGSYT NIKVTTPDDL LLAERILSED S
Sequence Positions
62-302, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
33,937 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

NCBI/Uniprot Data

NCBI Accession #
NP_001325418.1
NCBI GI #
1063699670
NCBI GenBank Nucleotide #
NM_001335124.1
NCBI GeneID
814779
NCBI Official Full Name
Nucleotide-diphospho-sugar transferases superfamily protein
NCBI Official Symbol
ISPD
NCBI Official Synonym Symbols
2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLTRANSFERASE; 4-DIPHOSPHOCYTIDYL-2C-METHYL-D-ERYTHRITOL SYNTHASE; ATMEPCT; MCT; T8K22.20; T8K22_20
NCBI Protein Information
Nucleotide-diphospho-sugar transferases superfamily protein
NCBI Summary
Encodes a protein with 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase activity. The enzyme has an absolute requirement for divalent cations (Mg2+ reaches the highest catalytic activity).
UniProt Gene Name
ISPD
UniProt Synonym Gene Names
MCT; MECT; MEPCT; AtMECT; AtMEPCT
UniProt Protein Name
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic
UniProt Synonym Protein Names
4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; AtMECT; AtMEPCT
UniProt Primary Accession #
P69834
UniProt Secondary Accession #
O64726; Q9LL91
UniProt Related Accession #
P69834
UniProt Comments
Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Is essential for chloroplast development and required for pigments and gibberellins biosynthesis.

For research use only, not for clinical use.