12-oxophytodienoate reductase 3 (OPR3), Recombinant Protein
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12-oxophytodienoate reductase 3 (OPR3), Recombinant Protein

Cat: RP00916
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 3 (OPR3)
Product Gene Name
OPR3 recombinant protein
Product Synonym Gene Name
OPR3
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MTAAQGNSNE TLFSSYKMGR FDLSHRVVLA PMTRCRALNG VPNAALAEYY AQRTTPGGFL ISEGTMVSPG SAGFPHVPGI YSDEQVEAWK QVVEAVHAKG GFIFCQLWHV GRASHAVYQP NGGSPISSTN KPISENRWRV LLPDGSHVKY PKPRALEASE IPRVVEDYCL SALNAIRAGF DGIEIHGAHG YLIDQFLKDG INDRTDQYGG SIANRCRFLK QVVEGVVSAI GASKVGVRVS PAIDHLDATD SDPLSLGLAV VGMLNKLQGV NGSKLAYLHV TQPRYHAYGQ TESGRQGSDE EEAKLMKSLR MAYNGTFMSS GGFNKELGMQ AVQQGDADLV SYGRLFIANP DLVSRFKIDG ELNKYNRKTF YTQDPVVGYT DYPFLAPFSR L
Sequence Positions
1-391, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
42,691 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
12-oxophytodienoate reductase

NCBI/Uniprot Data

NCBI Accession #
NP_001077884.1
NCBI GI #
145328276
NCBI GenBank Nucleotide #
NM_001084415.2
NCBI GeneID
815160
NCBI Official Full Name
oxophytodienoate-reductase 3
NCBI Official Symbol
OPR3
NCBI Official Synonym Symbols
11-REDUCTASE; 12-OXO-PHYTODIENOATE REDUCTASE; 12-OXOPHYTODIENOATE-10; AtOPR3; DDE1; DELAYED DEHISCENCE 1; F5K7.19; F5K7_19; oxophytodienoate-reductase 3
NCBI Protein Information
oxophytodienoate-reductase 3
NCBI Summary
Encodes a 12-oxophytodienoate reductase that is required for jasmonate biosynthesis. Mutants are male sterile and defective in pollen dehiscence. Shows activity towards 2,4,6-trinitrotoluene.
UniProt Gene Name
OPR3
UniProt Synonym Gene Names
DDE1; AtOPR3; OPDA-reductase 3
UniProt Protein Name
12-oxophytodienoate reductase 3
UniProt Synonym Protein Names
12-oxophytodienoate-10,11-reductase 3; AtOPR3; OPDA-reductase 3
UniProt Primary Accession #
Q9FUP0
UniProt Secondary Accession #
Q9LLD6; Q9SCY4; Q9XHD2; Q9ZQ01; B9DGR2
UniProt Related Accession #
Q9FUP0
UniProt Comments
Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).

For research use only, not for clinical use.