12-oxophytodienoate reductase 2 (OPR2), Recombinant Protein
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12-oxophytodienoate reductase 2 (OPR2), Recombinant Protein

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Cat: RP00926
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 2 (OPR2)
Product Gene Name
OPR2 recombinant protein
Product Synonym Gene Name
OPR2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEMVNAEAKQ SVPLLTPYKM GRFNLSHRVV LAPLTRQKSY GSVPQPHAIL YYSQRTSPGG FLIAEATGVS DTAQGYPDTP GIWTKEHVEA WKPIVDAVHA KGGIFFCQIW HVGRVSNRGF QPRRQAPISC TGKPIMPQMR ANGIDEARFT PPRRLSIEEI PGIVNDFRLA ARNAMEAGFD GVEIHGAHGY LIDQFMKDKV NDRTDEYGGS LQNRCKFALE VVDAVAKEIG PDRVGIRLSP FADYMESGDT NPEALGLYMV ESLNKYGILY CHMIEPRMKT VGEIAACSHT LMPMREAFKG TFISAGGFTR EDGNEAVAKG RTDLVAYGRW FLANPDLPKR FQLDAPLNKY NRSTFYTSDP VVGYTDYPSL ESTA
Sequence Positions
1-374, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
41,567 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
12-oxophytodienoate reductase

NCBI/Uniprot Data

NCBI Accession #
NP_177795.1
NCBI GI #
15223140
NCBI GenBank Nucleotide #
NM_106319.5
NCBI GeneID
844002
NCBI Official Full Name
12-oxophytodienoate reductase 2
NCBI Official Symbol
OPR2
NCBI Official Synonym Symbols
12-oxophytodienoate reductase 2; 12-OXOPHYTODIENOATE REDUCTASE 2; ARABIDOPSIS 12-OXOPHYTODIENOATE REDUCTASE 2; ATOPR2; F28O16.6; F28O16_6
NCBI Protein Information
12-oxophytodienoate reductase 2
NCBI Summary
Encodes one of the closely related 12-oxophytodienoic acid reductases. This enzyme is not expected to participate in jasmonic acid biosynthesis because during in vitro assays, it shows very little activity with the naturally occurring OPDA isomer. Shows activity towards 2,4,6-trinitrotoluene. Expressed predominately in root. Predicted to be a cytosolic protein.
UniProt Gene Name
OPR2
UniProt Synonym Gene Names
AtOPR2; OPDA-reductase 2
UniProt Protein Name
12-oxophytodienoate reductase 2
UniProt Synonym Protein Names
12-oxophytodienoate-10,11-reductase 2; AtOPR2; OPDA-reductase 2
UniProt Primary Accession #
Q8GYB8
UniProt Secondary Accession #
Q1LYX5; Q9ZR80
UniProt Related Accession #
Q8GYB8
UniProt Comments
Specifically cleaves olefinic bonds in alpha,beta-unsaturated carbonyls and may be involved in detoxification or modification of these reactive compounds. May be involved in the biosynthesis or metabolism of oxylipin signaling molecules. In vitro, reduces 9R,13R-12-oxophyodienoic acid (9R,13R-OPDA) to 9R,13R-OPC-8:0, but only poorly 9S,13S-OPDA, the natural precursor of jasmonic acid. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro and in vivo by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).

For research use only, not for clinical use.