Single-cell Solution - CD BioSciences

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Single-cell Solution

Single-cell Solution

Single-cell analysis is the study of genomics, transcriptomics, proteomics, metabolomics and cell interactions at the single-cell level, which allows the study of cell-to-cell variation within a cell population (organ, tissue, and cell culture).

Signaling pathways are integrated networks mediating cell communications and coordinate cellular processes including cell growth, differentiation, metabolism, stress response and apoptosis. The responses to signaling pathway perturbations through genetic or pharmacological manipulations can be highly heterogeneous across cells. Thus, studying signaling pathways in single-cell resolution is key to understand many biological processes such as drug resistance.

CD BioSciences offers a series of solutions for single-cell analysis to enable the study of heterogeneity across cells, profiling of signaling networks, characterization of gene functions, and MOA study of drugs.

Single-cell solutions

Solutions Based on Single-cell Technologies

CD BioSciences applies the following technologies to study signaling pathways at the single-cell level. Other applicable approaches can also be developed upon request by clients.

Single-cell solutions

Immunology-based Technologies

  • Flow Cytometry

    Flow cytometry is an immunology-based approach, which uses fluorophore-labeled antibodies to detect and quantify protein abundance in individual cells.

  • Mass Cytometry

    Mass cytometry is based on inductively coupled plasma time-of-flight mass spectrometry (ICP-TOF-MS), which uses metal isotope-tagged antibodies to label proteins or protein modifications in cells to achieve high-dimensional single-cell proteome profiling.

  • Immuno-sequencing

    REAP-Seq (RNA expression and protein sequencing) and CITE-Seq (cellular indexing of transcriptomes and epitopes by sequencing), in both of which cells are labeled with antibodies conjugated to DNA barcodes (AbBs), allow simultaneous detection of targeted proteins by single-cell sequencing and quantification of RNA transcriptomes in the same cells.

  • Lab-on-chip

    Lab-on-chip technologies, including single-cell barcode chips (SCBCs) and single-cell Western Blotting (scWB), enable the detection of low-abundance proteins in a single-cell resolution.

  • Microfluidics

    Microfluidics studies single cells in a 3D environment where the culture condition can be manipulated in a high-throughput manner, allowing fine time resolution and control of stimulus.

Single-cell solutions

Omics Technologies

  • Mass Spectrometry

    Single-cell mass spectrometry, such as SCoPE-MS (single-cell proteomics by mass spectrometry) and SCoPE2, enables the quantification of a large number of proteins in single cells.

  • Single-cell Sequencing

    For genome analysis, the development of whole genome amplification (WGA) allows the study of the genome at the single cell level to track changes in cell population and study the genetic evolution of cancer.

    For epigenome profiling, ATAC-seq (assay for transposase-accessible chromatin using sequencing) can be applied to measuring the accessibility of the genome region for transcription.

    The transcriptome can be analyzed using single-cell RNA sequencing, similar to single cell DNA sequencing, to profile whole-genome transcriptional regulation. Besides, recently established spatial transcriptomics approaches, such as fluorescent in situ sequencing (FISSEQ), multiplexed MERFISH, and spatial barcoding, not only can evaluate the whole-genome transcriptional activity, but also provide spatial information on RNA expression.

Single-cell solutions

Single-Cell Imaging

  • Fluorescence Imaging

    Fluorescence microscopy provides information on protein localization, tissue organization. It uses antibodies bound to fluorescent molecules to detect target proteins.

    Sequential imaging approaches, such as multi-epitope-ligand cartography (MELC), multiplex immunofluorescence (MxIF), cyclic immunofluorescence (CycIF), allow sequential imaging of the same specimen without influencing antigen abundance or tissue structure.

  • MS-based Imaging

    Imaging mass cytometry (IMC), a MS-based immunological imaging technology, can be applied to quantify proteins and protein modifications, and provide subcellular level spatial information.

    MALDI-based imaging mass spectrometry (MALDI-MSI), with optimized ionization efficiency, can be used to profile metabolome and lipidome, as well as proteome in single cells.

  • Live Cell Imaging

    Live cell imaging allows observing, tracking, and quantifying dynamic cellular processes and characterizing subcellular structures with high spatial and temporal resolution in single cells at their native physiological state. It can be coupled with FRET and reporter assays to study signaling pathways.

    Fluorescence resonance energy transfer (FRET), based on energy transfer between two proximate fluorophores, can be used to monitor the interaction of proteins in real-time.

    Activity-based reporters are proteins fluorescently-labeled, which move between cell compartments upon activation/deactivation. They can be used to monitor signaling activities in cells.

Features

CD BioSciences offers cost-effect, high quality and hassle-free Single-Cell Platform to our clients worldwide. We guarantee to deliver our products and results on time. Please feel free to contact us.

For research use only. Not intended for any clinical use.