Protein Characterization - CD BioSciences

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Protein Characterization

Protein Characterization

Recombinant proteins, peptides, and antibodies account for a significant portion of new drug approvals in recent years. Thus, it is of great importance to characterize protein products for drug development purposes or just for studying the properties of proteins to understand how they function in signaling pathways.

Protein characterization involves a series of extensive study covering a wide range of analytical methods and techniques to detect, isolate, purify proteins and characterize structures and physicochemical properties of proteins, which varies from protein to protein. It is of great challenge to characterize proteins, since they are large biomolecules with complex structures and sensitive to conditions like temperature and pH.

CD BioSciences has successfully adapted a broad range of cutting-edge technologies in biochemistry, biophysics, molecular biology, molecular medicine to provide clients with a portfolio of comprehensive protein-based solutions including protein identification and characterization.

Gene sequence

Our Services

Protein characterization involves creating a profile of proteins’ physical, chemical, and biological properties. With our advanced platform, we offer a set of quality control assays and analytical assays to help our clients study their proteins of interest and possibly discover and develop novel protein drugs.

CD BioSciences provides customer-tailored protein characterization services including but not limited to these shown below.

Quality Assessment Intact Molecular
Weight Analysis
  • LC/ES-MS (Liquid Chromatography with Electrospray-Mass Spectrometry) with Q-TOF (Quadrupole-Time of Flight);
  • MALDI-TOF/MS (Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry)
Purity and
Impurity Test
  • SDS-PAGE;
  • HPLC;
  • SEC (Size Exclusion Chromatography)
Concentration
Determination
  • Bradford Assay;
  • BCA Assay;
  • ELISA;
Aggregation
Analysis
  • SEC-MALS (Size Exclusion Chromatography with Multi-Angle Laser-Light Scattering);
  • SV-AUC (Sedimentation Velocity Analytical Ultra Centrifugation)
Stability
Evaluation
  • SV-AUC (Sedimentation Velocity Analytical Ultra Centrifugation)
Sequence Feature Analysis Primary Amino
Acid Sequencing
  • LC-MS/MS and Edman Degradation (N-terminal sequencing)
Peptide
Mapping
  • Enzyme Digestion and LC-MS/MS
N-terminal Sequencing
Analysis
  • LC-MS/MS and Edman Degradation (N-terminal Sequencing)
C-terminal Sequencing
Analysis
  • Carboxypeptidase Digestion and MS
Epitope
Mapping
  • Site-directed Mutagenesis;
  • High-throughput Shotgun Mutagenesis;
  • Array-based Oligo-peptide Scanning;
  • XL-MS (Cross-linking-coupled Mass Spectrometry);
  • HDX (Hydrogen–deuterium Exchange)
Charge Isoform
Pattern
  • icIEF (Imaging Capillary Isoelectric Focusing)
  • IEX (Ion Exchange Chromatography)
Structure Analysis Secondary
Structure
  • Circular Dichroism (CD) Spectroscopy, far-UV (190-250 nm);
  • Fourier Transform InfraRed (FT-IR) Spectroscopy;
  • Intrinsic Fluorescence Spectroscopy;
  • Extrinsic Fluorescence Spectroscopy;
  • NMR (Nuclear Magnetic Resonance)
Tertiary
 Structure
  • Circular Dichroism (CD) Spectroscopy, near-UV (>250 nm);
  • NMR (Nuclear Magnetic Resonance)
Protein Modifications Glycan
Profiling
  • MALDI-TOF/MS
  • LC-MS/MS
Phosphorylation
Analysis
  • Western Blot
  • LC-MS/MS
Acetylation
Analysis
  • Western Blot
  • LC-MS/MS
Ubiquitination
Analysis
  • Western Blot
  • LC-MS/MS
Other Post
Translational
Modification (PTM) Analysis
  • Western Blot
  • LC-MS/MS
  • etc

Features

CD BioSciences offers cost-effect, high quality and hassle-free protein characterization services to our clients worldwide. We guarantee to deliver our results on time. Please feel free to contact us.

For research use only. Not intended for any clinical use.