Phospho-PRAS40 (Thr356) Antibody - CD BioSciences


Phospho-PRAS40 (Thr356) Antibody

Phospho-PRAS40 (Thr356) Antibody


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100 µL Online Inquiry
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Target Information
Target Name AKT1S1
Gene Abbr. AKT1S1
Gene ID 84335
Full Name AKT1 substrate 1
Alias Lobe, PRAS40
Introduction Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH.
Application WB
Dilutions Western Blot (1:100-1:2000)
MW(KDa) 40
Reactivity D. melanogaster
Storage & Handling
Storage Buffer 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA and 50% Glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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