Phospho-ATF-2 (Ser490/Ser498) Antibody - CD BioSciences

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Phospho-ATF-2 (Ser490/Ser498) Antibody

Phospho-ATF-2 (Ser490/Ser498) Antibody

SPA-00621

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0.1 mL Online Inquiry
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Target Information
Target Name ATF-2
Gene Abbr. ATF2
Gene ID 1386
Full Name activating transcription factor 2
Alias CRE-BP1, CREB-2, CREB2, HB16, TREB7
Introduction The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2.ATF-7 is another member of the ATF/CREB family of leucine zipper proteins. Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 conjugated to KLH.
Usage
Application WB, IHC
Dilutions Western Blot (1:1000); Immunohistochemistry (1:10-1:500)
MW(KDa) 65, 75
Reactivity Human, Rat
Specificity Specific for ~74kDa ATF2 protein phosphorylated at Ser490/498. The also recognizes the phosphorylated ~54kDa splice form of ATF2.
Storage & Handling
Storage Buffer 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA and 50% Glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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