Mass Spectrometry Platform
Mass Spectrometry (MS) measures the mass‐to‐charge ratio of ions, which is widely used to identify unknown compounds, quantify known molecules, and determine their structure and chemical properties. It has been applied in many fields including biological and medical research.
In the study of signaling pathways, MS has been broadly used to characterize proteins and protein post-translational modifications (PTMs), determine protein interactions, and analyze metabolites.
Adapting the most advanced technologies, CD BioSciences offers a panel of MS-based solutions to enable broader and deeper analyses of your biological systems of interest.
Basic Principle of MS
A mass spectrometer is an instrument measuring the mass‐to‐charge ratio of ions. It contains at least three components: an ion source, a mass analyzer, and an ion detector.
- Ion Source - to ionize, and in some cases vaporize the sample.
- Mass Analyzer - to separate the ions according to their mass-to-charge ratio.
Examples: magnetic sector, quadrupole, ion trap, time-of-flight (TOF), and Fourier-transform ion cyclotron resonance (FTICR or FT).
- Ion detector – to detect and record the relative abundance of each ion.
Examples: Faraday cup, electron multiplier and the microchannel plate.
|Hard Ionization||Electron ionization (EI)|
|Soft Ionization||Electrospray Ionization (ESI)
Matrix-assisted Laser Desorption/Ionization (MALDI)
Electrospray Ionization (ESI)
Atmospheric-Pressure Chemical Ionization (APCI)
Desorption Electrospray Ionization (DESI)
MS can be categorized based on separation techniques they are combined with or applications, such as:
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Capillary Electrophoresis-Mass Spectrometry (CE-MS)
- Ion Mobility-Mass Spectrometry (IM-MS)
- Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)
- Ion Chromatography-Mass Spectrometry (IC-MS)
- Glow Discharge-Mass Spectrometry (GD-MS)
- Isotope Ratio-Mass Spectrometry (IR-MS)
Proteomics study the features of proteome in a biological system, which includes identification of novel proteins, detection of PTMs, determination of protein interactions, and analysis of protein structures.
CD BioSciences offers MS-based solutions to enable broader and deeper analyses into the proteome, including but not limited to protein identification and characterization, de novo amino acid sequencing, Ile/Leu determination, peptide mapping, terminal sequencing analysis, disulfide bond mapping, epitope mapping and isoform characterization.
Learn more about our Proteomics solutions.
Metabolomics and Lipidomics
Metabolomics study the metabolome of a cell, tissue or organism. Lipidomics, a subset of metabolomics, study the lipidome. Metabolome and lipidome are more complex than proteome, as metabolites are more diverse in terms of their chemical structure, and other properties.
CD BioSciences offers a panel of MS-based solutions to enable broader and deeper analyses into the metabolome and lipidome, including targeted or non-targeted metabolite profiling, metabolic flux analysis, and profiling all types of lipid species.
Learn more about our Metabolomics and Lipidomics solutions.
Glycomics is the study of the entire glycome, which includes analysis of glycan structures. The determination of the complete structure of oligosaccharides, formed by monosaccharides linked through glycosidic bonds, is more complex than protein's, because they are highly branched and with modifications.
CD BioSciences employs MS-based technologies (e.g. LC-MS) to perform glycan analysis, such as structural characterization, where the glycan part is cleaved from the target (glycoproteins, glycolipids) and subjected to determination of its monosaccharide sequence and its branching pattern, as well as determination of isomer position and the anomeric configuration of glycosidic bonds.
Oligonucleotides or oligomers are short DNA or RNA molecules. Oligonucleotides are characterized by the sequence of nucleotide residues (composed of a nitrogenous base, a ribose sugar and a phosphate group) and the covalent modifications on the residues. tRNAs, rRNAs, and small RNAs (microRNAs) are well-known oligonucleotides existing in a biological system.
CD BioSciences offers MS-based analytical techniques to identify modifications, and determine their structures and positions in oligonucleotides (e.g., m6A, m4C, m1A).
Learn more about our Epigenomics solutions.
Drug metabolism and pharmacokinetics (DMPK) analysis studies the absorption/administration, distribution, metabolism, and elimination/excretion (ADME) and toxicity properties of drugs, which involves the analysis using complex samples (e.g., blood, urine).
CD BioSciences employs MS-based technologies to study the pharmacokinetics of drugs, including sensitively detecting and accurately quantifying the metabolites in various types of samples.
Learn more about our DMPK analysis.
CD BioSciences offers cost-effect, high quality and hassle-free MS-based solutions to our clients worldwide. We guarantee to deliver our products and results on time. Please feel free to contact us.
For research use only. Not intended for any clinical use.