Epitope Mapping - CD BioSciences

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Epitope Mapping

Epitope Mapping

Epitope mapping is the process of experimentally identifying the binding site (epitope) of an antibody on its target antigen. It is instrumental in antibody drug development and antibody therapeutics.

Dysregulations of signaling transduction pathways usually lead to pathological or disease states. For example,  the mTORC1 pathway regulates many major cellular processes and is implicated in an increasing number of pathological conditions, including cancer, obesity, type 2 diabetes, and neurodegeneration. To find cures for diseases, proteins regulated the relevant pathways are potential targets.

CD BioSciences offers accurate, clear and directly applicable epitope definitions for your antibodies. With us, you can develop potent antibody drugs and protect your drugs with patents.

Epitope-Mapping

Features of Epitope Mapping Methods

Linear
(continuous)
Conformational
(discontinuous)
Time-efficient Low Cost
Site-directed mutagenesis ✓✓✓ ✓✓✓
High-throughput shotgun mutagenesis ✓✓✓ ✓✓✓
Array-based oligo-peptide scanning ✓✓✓ NO ✓✓✓ ✓✓✓
Cross-linking-coupled Mass Spectrometry (XL-MS) ✓✓✓ ✓✓✓ ✓✓
Hydrogen–deuterium Exchange (HDX) ✓✓✓ ✓✓✓ ✓✓

Epitopes are generally divided into two classes: linear and conformational. Linear epitopes are formed by a continuous sequence of amino acids, while conformational epitopes are discontinuous amino acids brought together upon three-dimensional protein folding.

CD BioSciences offers different epitope mapping methods to suit your need the best. With our advanced technology, we will achieve the timely delivery of the results.

Advantages of CD BioSciences Epitope Mapping Platform

Epitope Mapping

With extensive experience, we provide tailored consultations to work out the best way to achieve your goals.

Epitope Mapping

We offer multiple methods with broad applicability, so you can map linear or conformational epitopes to single amino acid resolutions.

Epitope Mapping

As a provider of high-quality reagents and services, we have a large library of antigens, including proteins, small-molecule chemicals, viruses and antibodies.

Epitope Mapping

We offer a variety of innovative solutions for in-depth analysis of epitopes for a large range of antibody formats and isotypes, including epitome-wide antibody cross-reactivity analysis, and antigen, epitope and mimotope discovery.

Applications

Epitope Mapping
  • Strengthening Intellectual Property (IP).
  • Comparing two antibodies with a similar epitope.
  • Classifying different antibodies into separate binding bins based on discovered epitopes.
  • Identifying essential and variable amino acid positions within an epitope.
  • Understanding of amino acid requirement for a cross-species or multi-strain reactivity analysis.

CD BioSciences offers a comprehensive epitope mapping service package to support your intellectual property (IP) claims, as well as to help you elucidate the mechanisms of binding for antibodies.

Features

CD BioSciences offers cost-effect, high quality and hassle-free epitope mapping services to our clients worldwide. We guarantee to deliver our results on time. Please feel free to contact us.

For research use only. Not intended for any clinical use.