Epigenomics - CD BioSciences

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Epigenomics

Epigenomics

Epigenomics is the systematic analysis of the epigenetic modifications (DNA and histone modifications) on the genetic material (DNA) of a biological system. These modifications “mark” the genome, control the expression of genes and can be passed down to the next generation. Different from genomes which are identical in all cells of an organism, epigenomes are cell type-specific.

Engaging multiple high-tech platforms, CD BioSciences offers powerful solutions to uncover the mystery of epigenomics, discover signaling pathways regulating epigenetic modifications and understand the functions of those modifications.

Epigenomics

DNA Methylation

DNA methylation refers to the reaction, in which methyl groups are tagged to DNA molecules, commonly at position C5 or N3 on cytosines and at position N6 on adenines. The reaction can be catalyzed either enzymatically by DNA methyltransferases (DNMTs) or chemically. The methyl groups can be removed by DNA demethylases in DNA demethylation.

DNA methylation is stable and inheritable. It directly regulates gene expression by affecting the interaction between DNA and proteins, which makes it essential in repression of transposable elements, regulation of tissue-specific gene expression, genomic imprinting, and X chromosome inactivation.

The profiling of DNA methylation includes the determination of the methylated regions on genome, the methylated DNA bases (A or C), and the methylated positions on the nucleobases.

DNA methylation

Solutions for DNA Methylation

DNA Methylation Detection

  • Methylated DNA Immunoprecipitation (MeDIP) - isolating methylated DNA fragments from input DNA, followed by DNA sequencing (MeDIP-seq) to determine the sequence of methylated DNA.
  • Mass Spectrometry (MS) - sensitively and reliably detecting DNA methylation, not informative about the sequence context.
  • Whole Genome Bisulfite Sequencing (WGBS) and Reduced Representation Bisulfite Sequencing (RRBS) - genome-widely detecting 5-methylcytosine (5 mC).

Writer/Eraser Identification

  • Identifying methyltransferases (writers) or demethylases (erasers) of DNA methylation.
  • Characterizing the methyltransferases or demethylases identified, in terms of their activity, structure, etc.

ChIP-seq

  • Determining the binding sites on the genome of certain DNA methyltransferases or demethylases. 

Histone Modification

Histones, found in eukaryotic cell nuclei, function to condense the DNA. Histone modifications are mostly covalent post-translational modifications (PTMs) to histone proteins, which regulate gene expression by altering chromatin structure and affecting interaction between DNA and proteins.

The PTMs known for histones include methylation, phosphorylation, acetylation, ubiquitylation, and SUMOylation, with examples shown down below.

Histone modifications

Solutions for Histone Modification

Histone Modification Detection

  • Detecting modifications involved in certain signaling pathways or related to the function of certain genes.

Histone Modifier Identification

  • Identifying enzymes catalyzing the histone modifications of interest.
  • Characterizing the modifiers, in terms of their activity, structure, etc.

ChIP-seq

  • Determining the chromatin region with certain histone modifications.
  • Determining the DNA sequence interacting with certain histone modifiers.

We also offer chromatin accessibility assays such as ATAC-Seq, MNase-seq, DNase-seq and FAIRE-seq to measure the accessibility of genome region for transcription. You may also find our PTM Enzymes solution helpful.

Features

CD BioSciences offers cost-effect, high quality and hassle-free epigenomics solutions to our clients worldwide. We guarantee to deliver our products and results on time. Please feel free to contact us.

For research use only. Not intended for any clinical use.