Customized Gene Engineering
Genes hold instructions of how the cells function. It is the basic physical and functional unit of heredity in a living organism. Manipulating gene expression allows scientists to analyze gene function and study the effect of genes on cellular activity or the phenotypes of an organism. In signaling pathway research, manipulations of gene expression are required for understanding how gene functions in the signaling transduction process.
As a leading provider of customized services, CD BioSciences offers a comprehensive solution to help you manipulate the expression of your genes of interest. With cutting-edge gene editing technologies, we will make any gene modification you want in the organism you specify.
To help scientists in their quest to understand how genes affect phenotype, we offer a comprehensive genome engineering service portfolio with advanced technologies, including the popular CRISPR system.
- CRISPR Knockout
Gene knockout is primarily used to dissect the function of genes. Comparing the phenotypes of gene knockout organisms to wildtype allows researchers to understand the effect of gene loss.
Disable gene by manipulating the DNA sequence to introduce a frameshift, which abolishes gene function.
- CRISPR Knockin
Gene knockin refers to the one-for-one substitution of DNA sequence in a genetic locus or the insertion of DNA sequence not found within the locus.
With CRISPR/Cas9, our service introduces specific mutations, DNA sequences or tags to genes via homology directed repair of the lesion cut by Cas9.
CRISPR activation (CRISPRa) technique provides a complementary approach to gene overexpression.
With dCas9 (a catalytically dead Cas9) fused with a transcriptional effector (e.g. KRAB domain) to activate target gene expression, our service designs gRNA (guide RNA) and generates stable cell lines with your gene expression upregulated. It can also be applied to genome wide activation.
CRISPR interference (CRISPRi) technique provides a complementary approach to RNA interference (RNAi). Different from RNAi’s regulation on the mRNA level, CRISPRi regulates gene expression on the transcriptional level.
With dCas9 (a catalytically dead Cas9) fused with a transcriptional effector (e.g. VPR, SunTag) to repress target gene expression, our service designs gRNA (guide RNA) and generates stable cell lines with your gene expression downregulated.
Overexpression of genes is critical for many applications, such as living cell imaging for the determination of protein localization and protein purification for structure analysis.
Our service generates lentiviral-based stable cell lines overexpressing your gene of interest.
- shRNA Knockdown
Knockdown of gene expression is a fundamental technology to study gene function, especially when knockout of a gene is lethal.
Our service generates lentiviral-based stable cell lines expressing shRNA (short hairpin RNA) to downregulate the expression of your gene of interest.
Please note that our services are not limited to gene modification in cell lines. We also serve to manipulate gene expression in bacteria, yeast, plant and animal models (e.g. C. elegans, fruitfly, zebrafish, mouse, rat).
CD BioSciences offers cost-effect, high quality and hassle-free gene engineering services to our clients worldwide. We guarantee to deliver our products on time. Please feel free to contact us.
For research use only. Not intended for any clinical use.