Customized Gene Cloning
In the area of life science research such as studying cellular signaling pathways, analysis of gene expression and function is one of the most important aspects.
To help you advance your discovery, CD BioSciences offers different cloning options to meet your needs. You can have any sequence to be inserted in any vector you want.
In molecular genetics, an open reading frame (ORF) is the part of a reading frame that has the ability to be translated. An ORF is a continuous stretch of codons that begins with a start codon (usually AUG) and ends at a stop codon (usually UAA, UAG or UGA).
Types of Cloning
- ORF cDNA Cloning
Genes often have different ORFs. The wrong version of ORFs might result in non-functional proteins. We provide fully sequenced ORFs upon your request.
- ORF cDNA mutant cloning
We create custom point mutations, insertions, and deletions, from any wild type sequences. You are free from expensive, unpredictable mutagenesis kits and protocols.
- Promoter Assembly and Cloning
Building a reporter stain? Studying genes transcription activity? We can help you create your own composite promoter by combining the enhancer, core promoter and 5’UTR.
- DNA Sequence Synthesis
We will synthesize any sequences upon your request, including repeated sequences, GC-rich or AT-rich sequences, toxic sequences.
Customization of Your Plasmid
You can have your genes inserted in our vector, or the vector you provided.
All you need to do is provide us with your gene of interest or DNA sequence.
There are numerous tagging options for you. Tell us which tag you want for your protein at N- or C- terminus.
- Epitope tags: Flag, HA, His, Myc, Fc, GST, etc.
- Fluorescent tags: GFP, RFP, BFP, YFP, Cyan, Far-Red, etc.
- Mammalian selection markers: Neomycin, Promycin, Blasticidin, Hygromycin, etc.
CD BioSciences offers cost-effect, high quality and hassle-free gene cloning services to our clients worldwide. We guarantee to deliver our products on time. Please feel free to contact us.
For research use only. Not intended for any clinical use.