CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeat DNA sequences. The most widely used type II CRISPR/Cas9 genome engineering system uses CRISPR-associated protein 9 (Cas9) which is a nuclease derived from the bacterial species Streptococcus pyogenes, sgRNA which is contained in crRNA, tracrRNA which binds to crRNA to form an active complex, and an optional DNA repair template.
Once a mechanism to defend against viruses and foreign DNA in bacteria, CRISPR system has now become an indispensable tool in biological and medical research. It is widely used for genome editing and with applications beyond that.
Adapting the most advanced CRISPR technologies, CD BioSciences offers a series of solutions based on CRISPR/Cas9 systems, including gene editing, gene regulation and high-throughput screening to study signaling pathways and characterize diseases.
Our CRISPR-based Technologies
Due to its robustness and flexibility, CRISPR has become a versatile tool with applications in not only genome editing, but also epigenome and chromatin manipulation.
CRISPR system is a simple yet powerful tool for gene editing, where the Cas9 nuclease is guided by a single-guide RNA (sgRNA) to the target sequence with protospacer adjacent motif (PAM) and make cleavage, therefore generating a double-strand break (DSB) in the genome. The DBS can be repaired by non-homologous end joining (NHEJ) which CRISPR KO (knockout) is based on or homology directed repair (HDR) which CRISPR KI (knockin) is based on.
Gene regulation by CRISPR system uses a proteolytically deactivated Cas9 (dCa9) fused with a transcriptional effector (e.g., VPR, SunTag, SAM) to bind target DNA, in which the expression of the target gene is activated (CRISPRa) or repressed (CRISPRi).
Epigenome describes heritable histone post-translational modifications, DNA modifications and other chromatin features associated with regulatory elements in the genome. In epigenome editing, dCas9 recruits various epigenetic writers and erasers to a specific locus and makes changes that affect gene expression.
Chromatin Imaging and Manipulation
Chromatin imaging uses dCas9 fused to different fluorescent proteins to target telomeres and centromeres, which allows tracking of native chromatin loci throughout the cell cycle in live cells. Researchers also use dCas9 to achieve targeted and robust manipulation of chromatin structure and DNA loop formation.
Applications of Our CRISPR-based Technologies
- Performing targeted gene knockout or knockin using CRISPR/Cas9 system.
- Performing targeted gene activation (CRISPRa) or inactivation (CRISPRi) using CRISPR/dCas9 system.
Learn more about our Customized Gene Engineering services.
- High-throughput CRISPR KO, CRISPRi (interference) and CRISPRa (activation) screening with pooled sgRNA libraries to manipulate gene expressions genome-widely or in specific pathways.
Disease Model Generation
- In vivo genome editing in cells or organisms to create models of diseases by inactivating individual genes or eliminating an entire chromosome at the desired time point of their development.
Learn more about our Model Creation service.
- Developing potential CRISPR-based therapeutics as treatment for diseases, especially diseases with genetic causes, including cancers, immune system disorders.
Advantages of CRISPR-based Systems
CD BioSciences offers cost-effect, high quality and hassle-free CRISPR-based solutions to our clients worldwide. We guarantee to deliver our products and results on time. Please feel free to contact us.
For research use only. Not intended for any clinical use.