Cleaved Caspase-8 (Asp391) Antibody - CD BioSciences


Cleaved Caspase-8 (Asp391) Antibody

Cleaved Caspase-8 (Asp391) Antibody


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Target Information
Target Name Caspase-8
Gene Abbr. CASP8
Gene ID 841
Full Name caspase 8
Alias ALPS2B, CAP4, Casp-8, FLICE, MACH
Introduction Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10. Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 18C8
Immunogen Synthetic peptide corresponding to residues adjacent to Asp391 of human Caspase-8.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human Caspase-8.
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 18, 41, 43
Reactivity Human
Sensitivity Endogenous
Specificity This antibody detects human Caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone. A band of unknown origin is detected at 25 kDa in some cell lines.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.