Cell Line Engineering
Generation of cell lines is time-consuming and complex, but inevitable for investigating the mechanisms of signaling pathways.
CD BioSciences is a leading provider of custom cell line engineering services. With our cutting-edge technology, we will make any modification you want in the cell line you specified.
Types of Cell Line Engineering
Overexpression of proteins is critical for many applications, such as live-cell imaging for the determination of protein localization and protein purification for structure analysis. CD BioSciences offers the generation of stable cell lines overexpressing your protein of interest upon your request.
Knockdown of gene expression is a fundamental technology to study gene function, especially when the knockout of a gene is lethal. Short hairpin RNAs (shRNAs) can be integrated into the chromosome to generate stable cell lines. Using our virus packaging system, we will generate shRNA knockdown cell lines for you.
Recently, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems have turned into one of the most popular genome editing tools. Using the CRISPR system, we will generate gene knockin or knockout in your cell line in favor.
Primary Cell Immortalization
Primary cell lines undergo a limited number of proliferations because of replicative senescence. Immortalization of primary cells is commonly used for research studying cellular biology. We offer multiple approaches to cell immortalization including the most popular ones as SV40T and hTERT.
CD BioSciences offers cost-effect, high quality and hassle-free cell line engineering services to our clients worldwide. We guarantee to deliver our products on time. Please feel free to contact us.
For research use only. Not intended for any clinical use.